大黄素对实验性自身免疫性心肌炎大鼠IFN-γ及IL-17水平的影响

The Effects of Emodin on the Levels of IFN-γ,IL-17 in Experimental Autoimmune Myocarditis Rats

目的探讨大黄素对自身免疫性心肌炎大鼠IFN-γ及IL-17的影响,及其对大鼠的心肌保护作用。方法 6周龄雄性Lewis大鼠24只随机分为3组(8只/组):自身免疫性心肌炎(EAM)组、大黄素(Emodin)组和对照(Control)组。于实验0 d及7d,EAM组和Emodin组用心肌肌球蛋白/完全弗氏佐剂免疫,而对照组仅用完全弗氏佐剂假性免疫。Emodin组于0 d起,每日给予50 mg大黄素灌胃治疗,共21 d,而EAM组和对照组每日给予蒸馏水灌胃。21 d时处死大鼠,心肌组织行HE染色及Masson染色,光镜下评价心肌组织炎细胞浸润及纤维化程度;Western blot法检测心肌组织IFN-γ及IL-17蛋白的表达水平;Elisa法检测血清中IFN-γ及IL-17的水平。结果与EAM组相比,大黄素显著减轻了Emodin组心肌组织中的炎性细胞浸润及纤维化改变。EAM组和Emodin组血清及心肌组织中IFN-γ和IL-17的水平明显高于Control组(P<0.05)。而Emodin组上述指标均明显低于EAM组(P<0.05)。结论大黄素可以降低IFN-γ和IL-17的水平,对EAM大鼠的心肌起到保护作用。

Objective To investigate the effects of emodin on the IFN-γ,IL-17 expression in EAM rats and its protective effect on myocardial.Methods Male Lewis rats（n = 24,6 weeks old）were randomLy assigned into three groups（8 animals/group）：experimental autoimmune myocarditis（EAM）group,emodin（Emodin）group and control（Control）group.On days 0 and 7,EAM group and Emodin group were immunized with myosin/Freund′s complete adjuvant（FCA）,the Control group was immunized with FCA alone.Emodin group was intragastric administrated with emodin 50 mg/kg per day for 3 weeks,while EAM group and Control group were given distilled water.All rats were sacrificed on day 21.Myocardial tissue were stained with hematoxylin and eosin（HE）and Masson′s,and analyzed under a light microscopy to evaluated the degree of inflammatory cell infiltration and fibrosis;Western blot analysis assay was performed to detect IFN-γ and IL-17 protein expression levels in myocardial tissue;Serum concentration of IFN-γ and IL-17 was measured with ELISA kits.Results Emodin significantly reduced inflammatory cells infiltration and fibrosis in Emodin group compared to EAM group.IFN-γ and IL-17 levels in serum and myocardial tissue were markedly increased in EAM group and Emodin group compared to the Control group（P 0.05）.Moreover,the above mentioned indexes in Emodin group were obviously lower than EAM group（P 0.05）.Conclusion Emodin can reduce the levels of IFN-γ and IL-17,and protect the myocardial tissue in EAM rats.