钙结合蛋白S100A1在卵巢癌中的表达及意义

Expression of S100A1 protein in ovarian carcinoma

目的:探讨钙结合蛋白S100家族中的S100A1在正常卵巢以及卵巢癌中的表达及临床意义。方法:收集临床手术标本,包括正常卵巢20例,卵巢浆液性囊腺癌30例,非浆液性卵巢癌18例。用免疫组化的方法测定标本中S100A1蛋白的表达,分析其表达水平与临床病理参数之间的关系,探讨其临床意义。结果:S100A1在卵巢癌组织中表达高于正常卵巢组织(P<0.05),两者免疫染色吸光度分别为5.78±0.39和1.82±0.18;在浆液性卵巢癌组中的表达水平明显高于非浆液性卵巢癌(P<0.05),两者免疫染色吸光度分别为5.98±0.28和5.45±0.15;浆液性卵巢癌中S100A1的表达水平与卵巢癌的病理分级呈正相关(P<0.05),但与卵巢癌的手术病理分期、淋巴结转移与否无关(P>0.05);非浆液性卵巢癌中S100A1的表达水平与肿瘤的手术病理分期、病理分级以及淋巴结转移均无关(P>0.05)。结论:①S100A1与卵巢癌的发生相关;②在浆液性卵巢癌和非浆液性卵巢癌中S100A1的作用机制不同;③S100A1与浆液性卵巢癌恶性程度相关,高表达则恶性程度高;④S100A1可能是癌基因,高表达提示卵巢癌。

Objective： To explore the expression level of S100A1 protein, a calcium binding protein, in normal ovaries and ovarian cancer samples. Methods ： The tissues were sampled from 20 women with normal ovary, 30 women with serous ovarian carcinoma, and 18 women with non serous ovarian cancer. The expression level of S100A1 protein in specimens collected was evaluated using the immunohistochemical method. And the relationship between its expression and clinicopathologic parameters was analyzed. Results ： The expression of S100A1 protein in the ovarian cancer sample （5.78 ± 0.39 ） was higher than that of normal ovary （1.82±0. 18, P 〈 0.05）. And it was higher in the serous ovarian carcinoma sample （5.98 ±0. 28） than that in the non serous ovarian cancer one （5.45±0.15, P 〈0.05）. The expression level of S100A1 protein in the serous ovarian carcinoma sample had positive correlation with the pathological grade of ovarian cancer （ P 〈 0.05 ）, but had no correlation with the surgical pathological stage and lymph node metastases of ovarian cancer（ P 〉 0.05）. And no cor relations were found between the expression level of S100A1 protein in the serous ovarian carcinoma sample and the surgical pathological stage, the pathologic grade and lymph node metastases of ovarian cancer （P 〉 0.05）. Conclusion： S100A1 pro tein is related with the occurrence of ovarian cancer. Difference is found in the mechanism of S100A12 between non serous and serous ovarian cancer. The expression level of S100A1 protein has positive correlation with the malignancy of serous ovari an cancer. S100A1 maybe an oncogene, and ovarian cancer could be suggested when abnormal expression level of S100A1 protein occurs