GM_1对实验性脑缺血再灌注损伤的作用

The effects of monosidoltetrahexosylganglioside(GM_1) on experimental cerebral ischemia and reperfusion damage

目的 研究单唾液酸四己糖神经节苷脂 (GM1 )对脑缺血再灌注损伤的作用。方法 采用大鼠全脑缺血再灌注动物模型(4VO) ,测定观察假手术组 (对照组 )、脑缺血 30 min再灌注 60 min生理盐水处理组、GM1 处理组脑海马组织线粒体钙 (MCa)、钙调素(Ca M)含量改变 ,以及缺血 30 min再灌注 4 d脑海马 CA1 区普通病理和超微病理改变。结果 脑缺血 30 min再灌注 60 min海马组织MCa、Ca M含量显著性升高 (P<0 .0 1 ) ,脑缺血 30 min再灌注 4d海马 CA1 区神经元呈现严重缺血、坏死病理改变 ,GM1 处理后则上述结果明显好转。结论 GM1 对脑缺血再灌注损伤具有保护作用。

o study the GM 1 effects on cerebral ischemia and reperfusion damage.Methods With global cerebral ischemia and reperfusion rat model(4VO),the hippocampic mitochondria calcium(MCa),calmodulin(CaM) contents at sham group,cerebral ischemia 30 min reperfusion 60 min group treated by normal saline(NS) and GM 1(10 mg/kg IP),were observed.and the pathologic changes of hippocampic CA 1 district after 4 days reperfusion followed 30 min ischemia GM 1 10 mg/kg IP,daily for 4 days were also studied.Results The hippocampic MCa,CaM contents were significantly higher on cerebral ischemia and reperfusion group than that in sham group( P <0 01),the hippocampic CA 1 district neuron showed obvious pathologic damage.The levels of MCa,CaM in GM 1 treated group were significantly lower than that of the control group with NS( P <0 01),and the pathologic changes were improved in GM 1 group.Conclusions GM 1 with cerebral protective effect on cerebral ischemia and reperfusion damage were confirmed.