异氟醚对原代培养的肺泡Ⅱ型上皮细胞合成表面活性物质的影响

Effect of isoflurane on pulmonary surfactant synthesis in cultured primary alveolar type Ⅱ cells

目的 通过原代培养的正常及受H2 O2 损伤的肺泡Ⅱ型上皮细胞 (ATⅡcell) ,探讨异氟醚 (Iso)对肺泡Ⅱ型上皮细胞合成表面活性物质的影响。方法 肺泡Ⅱ型上皮细胞分离、纯化、培养 32h后 ,在正常细胞或 75 μmol·L-1H2 O2 处理细胞的培养液内加入Iso 0 2 8、2 8mmol·L-1,继续培养 2h。分别通过MTT法检测肺泡Ⅱ型上皮细胞增殖功能 ,3H 胆碱参入法测其合成表面活性物质的功能。结果 Iso降低正常肺泡Ⅱ型上皮细胞合成表面活性物质的功能并加重H2 O2 引起的肺泡Ⅱ型上皮细胞表面活性物质合成功能的降低 ,而Iso对正常及受 (H2 O2 )损伤的ATⅡcell增殖功能均无显著影响。结论 Iso可以降低培养的肺泡Ⅱ型上皮细胞合成表面活性物质的功能 ,尤其是在过氧化环境中。

AIM To determine the effect of isoflurane(Iso) on pulmonary surfactant(PS) synthesis in cultured primary ATⅡ cells. METHODS ATⅡ cells were isolated from adult rat lungs and used for the experiments after 32 h in primary culture. Iso 0.28 and 2 8 mmol·L -1 was added into the media of normal and H 2O 2 (75 μmol·L -1 )-treated cells, and the cells were further incubated for 2 h. The cell proliferation was measured with MTT method and PS synthesis with 3H-choline chloride incorporation. RESULTS Iso had no effect on the proliferation of ATⅡ cells, but markedly decreased PS synthesis in normal alveolar type Ⅱ cells, and aggravated the decrease of PS synthesis induced by H 2O 2. CONCLUSION Iso may decrease PS synthesis of alveolar type Ⅱ cells in vitro , and aggravate the damage of the cells under peroxidation condition.