摘要
目的 研究尼莫地平 (Nim)对脑缺血诱发细胞凋亡的抑制作用。方法 大鼠不完全脑缺血采用双侧颈总动脉(CCA)结扎法 ;细胞凋亡采用Tunel法测定 ;DNA片端含量用二苯胺试剂法测定。结果 结扎双侧CCA可显著诱导脑皮质细胞凋亡 ;2mg·kg-1Nim可显著减少脑皮质中细胞凋亡数 ;1mg·kg-1、2mg·kg-1Nim可抑制脑缺血诱导DNA片端的增多。结论 Nim对缺血诱导的脑皮质细胞凋亡有抑制作用。
AIM To study the inhibitory effect of nimodipine on apoptosis induced by cerebral ischemia. METHODS Incomplete cerebral ischemia was made by ligating bilateral common carotid arteries(CCA) in rats. The apoptosis was determined by terminal deoxy-nucleotidyl transforase-mediated dUTP-digoxigenin nick end-labeling(Tunel) method. The DNA fragmentation analysis was measured with the diphenylamine reagent method. RESULTS Ligting of bilateral CCA markedly produced apoptotic cell in cerebral cortex. Nim 2 mg·kg -1 significantly decreased the numbers of apoptotic cells in cortex; Nim 1 mg·kg -1 , 2 mg·kg -1 also inhibited the increase of DNA fragmentation induced by cerebral ischemia. CONCLUSION Nim has inhibitory effect on ischemia-induced apoptosis of cerebral cortex.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2000年第5期542-544,共3页
Chinese Pharmacological Bulletin
基金
安徽省教委资助!课题
No 97JL10 72
