摘要
应用Northern斑点杂交方法研究氯化镉对大鼠肝细胞原癌基因c -myc表达的影响。结果表明 ,5 μmol/kg、10 μmol/kg、2 0 μmol/kg氯化镉腹腔一次注射后 ,大鼠肝细胞c-mycmRNA较对照组明显增多 ,用免疫组化技术在大鼠肝细胞中检测到c -myc蛋白。结果提示 ,氯化镉可以引起在体大鼠肝细胞c-myc表达增强。
The research studied the effects of cadmium chloride on proto-oncogene c-myc expression in rat liver cells with Northern Dot Hybridization. The results showed: at the dose of 5μmol/kg, 10μmol/kg,20μmol/kg, cadmium chloride can induce proto-oncogene c-myc expression obviously and c-myc protein can be detected in rat liver cells with immunohistochemistry. The research infers that at certain dose,cadmium can induce proto-oncogene c-myc expression in rat liver cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第11期995-996,共2页
Chinese Journal of Public Health
基金
国家自然科学基金
