+Gz重复暴露大鼠脑组织中IL-1β和TNF-αmRNA表达变化

Changes of mRNA Expression of IL-1β and TNF-α in Rat Brains after Repeated Exposures to +Gz.

目的了解反复高 +Gz暴露后大鼠脑组织中IL 1 β和TNF αmRNA表达变化。方法 2 0只SD大鼠随机分成对照组、+Gz重复暴露后 3 0min、6h、2 4h和 48h五组 ,每组 4只。对照组大鼠G值为 +1Gz ,实验组大鼠在动物离心机上经历了 3次 +1 4Gz/45s(两次间间隔 3 0min)作用。分别于暴露后 3 0min、6h、2 4h和 48h处死大鼠取脑 ,提取总RNA ,用半定量反转录聚合酶链反应 (RT -PCR)检测方法检测大鼠脑组织中IL 1 β和TNF αmRNA表达水平。结果 +Gz暴露后 3 0min、6h和 2 4h大鼠脑组织IL 1 β和TNF αmRNA均有升高 ,但 48h时均恢复正常。结论反复高 +Gz暴露可刺激大鼠脑组织内IL 1 β和TNF αmRNA表达 ,它们在 +Gz所致脑损伤中起一定的作用 。

Objective To study changes of mRNA expression of IL 1β and TNF α in rat brains after repeated exposures to +Gz. Method Twenty concious SD rats served as the subjects. They were randomly divided into 5 groups. Using an animal centrifuge, control rats (n=4) were exposed to +1 Gz and experimental rats (n=16) were exposed to +14 Gz three times, each for 45 s with 30 min interval in between. The rat brains were taken 30 min, 6 h, 24 h and 48 h after the last centrifuge run and total RNA was isolated. mRNA expression levels of IL 1β and TNF α in rat brain were measured by semi quantitative reverse transcription polymerase chain reaction (RT-PCR). Result mRNA expression levels of IL 1β and TNF α in rat brains taken 30 min, 6 h and 24 h after repeated +Gz exposures were significantly higher than those in control rats, but returned to normal after 48 h. Conclusion It suggested that mRNA expressions of IL 1β and TNF α in rat brains can be stimulated by repeated +Gz exposures and the increased expression of IL 1β and TNF α may play a role in the pathologic course of brain damage induced by+Gz exposures, but the damage is reversiable.