艾拉光动力治疗对兔耳增生性瘢痕组织中MMP-2、MMP-9、MMP-13及TIMP-1的mRNA水平的影响

The effects of ALA-PDT on the expression of mRNA of MMP-9,MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears

目的:研究艾拉光动力治疗对兔耳增生性瘢痕中基质金属蛋白酶-2信使核糖核酸(MMP-2mRNA)、基质金属蛋白酶-9信使核糖核酸(MMP-9mRNA)、基质金属蛋白酶-13信使核糖核酸(MMP-13mRNA)和基质金属蛋白酶组织抑制剂-1信使核糖核酸(TIMP-1mRNA)的变化,探讨艾拉光动力治疗对兔耳增生性瘢痕的初步治疗机制。方法:将实验动物分为正常组、阴性对照组、高浓度艾拉光动力治疗组、低浓度艾拉光动力治疗组、光动力治疗组,对高浓度艾拉光动力治疗组、低浓度艾拉光动力治疗组、光动力治疗组分别进行干预治疗,1次/周,共3次,于治疗后1月、2月和3月采集样本,进行RT-PCR实验。结果:治疗组中MMP-2、MMP-9、MMP-13的mRNA表达水平在艾拉光动力治疗后1月、2月和3月的增生性瘢痕表达要明显高于阴性对照组,差异具有显著性(P?0.01),而治疗组中TIMP-1的mRNA表达水平要明显低于阴性对照组,差异具有显著性(P?0.01);高浓度治疗组与低浓度治疗组之间比较有差异(P?0.05)。结论:艾拉光动力治疗可改变兔耳增生性瘢痕中的MMP-2mRNA、MMP-9mRNA、MMP-13mRNA和TIMP-1mRNA的表达,使MMPs/TIMP-1之间的比例上调。

Objective： To investigate the effects of ALA-PDT treatment on the expression of mRNA of MMP-2, MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model of rabbits＇ ears, and analysis the treatment mechanism of ALA-PDT treatment to HS of rabbit ears. Methods： We divided the test animals into normal control, negative control, high concentration ALA-PDT, low concentration ALA-PDT and PDT groups. The latter three groups received PDT treatment once a week for 3 weeks. The specimens were collected respectively 1, 2 and 3 months after PDT to used for RT-PCR test. Results： 1, 2 and 3 months after PDT, the mRNA of MMP-2 MMP-9 and MMP-13 level in HS tissue of three treatment groups were significantly higher than those in the negative group （P〈0.01）, and the mRNA of TIMP-1 level of three treatment groups were significantly lower than those in the negative group （P〈0.01）. There were also significant difference between high concentration group and low concen- tration group （P〈0.05）. Conclusion： ALA-PDT treatment can change the expression of the mRNA of MMP-2, MMP-9, MMP-13 and TIMP-1 of the tissues of hypertrophic scar of rabbits＇ ears, resulting in the proportion be- tween the MMPs/TIMP-1 be up-regulated.