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儿童血清肺炎支原体抗体IgM三种检测方法比较 被引量:5

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摘要 目的:探讨儿童血清肺炎支原体抗体IgM最佳检测方法。方法:选择疑似肺炎支原体(MP)感染的患儿100例,100份血清标本同时采用酶联免疫吸附法、间接免疫荧光法和胶体金法三种方法进行平行检测,比较诊断符合率等有代表性的指标。结果:酶联免疫吸附法(符合率93%)和间接免疫荧光法(符合率92%)两者间检测血清抗MP无显著性差异,而两种方法与胶体金法(符合率80%)比较均有显著性差异。结论:儿童肺炎支原体抗体IgM推荐采用间接免疫荧光法来诊断肺炎支原体感染。
作者 刘琦
出处 《陕西医学杂志》 CAS 2013年第8期1078-1079,共2页 Shaanxi Medical Journal
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  • 1俞善昌.有关支原体感染的几个问题[J].实用儿科杂志,1993,8(3):209-210. 被引量:208
  • 2曹玉璞.小儿肺炎支原体感染的诊断与治疗[J].中国实用儿科杂志,1995,10(6):339-340. 被引量:172
  • 3夏邦世,吴金华.Kappa一致性检验在检验医学研究中的应用[J].中华检验医学杂志,2006,29(1):83-84. 被引量:212
  • 4苏爱芳,梁世鹏,孙旭红.支原体肺炎48例误诊分析[J].实用诊断与治疗杂志,2007,21(5):396-397. 被引量:10
  • 5Martinez M A, Ruiz M, Zunino E, et al. Detection of Mycoplasma pneumoniae in adult community-acquired pneumonia by PCR and serology [J]. J Med Microbiol, 2008,57(12) : 1491-1495.
  • 6Kashyap B, Kumar S, Sethi G R, et al. Comparison of PCR, culture & serological tests for the diagnosis of Mycoplasma pneumoniae in community-acquired lower respiratory tract infections in children [J ]. Indian J Med Res,2008,128(2) : 134-139.
  • 7王淑娟.实验诊断学[M].北京:北京医科大学、中国协和医科大学联合出版社,2001:260-261.
  • 8李之桂,范明远.感染症免疫诊断技术[M].北京:科学出版社,2009:254-257.
  • 9Tamura A, Matsubara K, Tanaka T, et al. Methylprednisolone pulse therapy for refractory Mycoplasma pneumoniae pneumonia in children[J]. Infect, 2008, 57(3): 223-228.
  • 10Kathleen AT, Kelley CC, Jonas MW, et al. Comparison of nucleic acid extraction methods for the detection of Myeoplasma pneumoniae[J]. Diagn Microbiol Infect Dis, 2009, 65(4) : 435- 438.

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  • 1Loens K, Goossens H, Ieven M. Acute respiratory infection due to Mycoplasma pneumoniae : current status of diagnostic methods [ J ]. Eur J Clin Microbiol Infect Dis, 2010, 29(9) : 1055 -1069.
  • 2Shimizu T, Kida Y, Kuwano K. Cytoadherence -dependent induc- tion of inflammatory responses by Mycoplasma pneumoniae [ J 1. Im- munology, 2011, 113(1): 51 -61.
  • 3Montagnani F, De Paolis F, Beghetto E, et al. Use of recombinant chimeric antigens for the serodiagnosis of Mycoplasma pneumoniae in- fection[J]. Eur J Clin Microbiol Infect Dis, 2010, 29(11 ): 1377 - 1386.
  • 4Talkington DF, Shott S, Fallon MT, et al. Analysis of eight commer- cial enzyme y tests for detection of antibodies to Myco- plasma pneumoniae in human serum[ J]. Clin Diagn Lab Immunol 2004, 11'(5): 862-867.
  • 5胡元生,温和,周银娣,李从磊.三种肺炎支原体检测方法的比较[J].临床输血与检验,2008,10(1):9-11. 被引量:25
  • 6Nelson,G.E.,Aguon,A.,Valencia,E,et al.Epidemiology of a mumps outbreak in a highly vaccinated island population and use of a third dose of measles-mumps-rubella vaccine for outbreak control-Guam 2009 to 2010[J].The Pediatric infectious disease journal,2013,32(4):374-380.
  • 7Smith-Norowitz,T.A.,Silverberg,J.I.,Kusonruksa,M,et al.Asthmatic children have increased specific anti-mycoplasma pneumoniae Ig M but not Ig G or Ig E-Values independent of history of respiratory tract infection[J].The Pediatric infectious disease journal,2013,32(6):599-603.
  • 8Gilliland,S.M.,Jenkins,A.,Parker,L,et al.Vaccine-related mumps infections in Thailand and the identification of a novel mutation in the mumps fusion protein[J].Biologicals:Journal of the International Association of Biological Standardization,2013,41(2):84-87.
  • 9施文平,申惠国,吴敏,谈逸云,杜艳,赵黎芳,席韵,倪政.上海市闵行区2005-2007年学校呼吸道传染病疫情特征分析[J].中国学校卫生,2008,29(12):1125-1126. 被引量:25
  • 10钱月芳,唐进,陆敏.肺炎支原体的3种实验室检查方法对照分析[J].临床肺科杂志,2009,14(11):1554-1554. 被引量:6

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