一种新型的基于普鲁士蓝/石墨烯/酪氨酸酶/空壳金修饰的双酚A传感器

A novel biosensor based on prussian blue/graphene/tyrosinase/hollow gold nanoparticles modified glassy carbon electrode for determination of bisphenol A

制备了一种新型的多层修饰的基于普鲁士蓝(PB)、石墨烯、酪氨酸酶(Tyr)和空壳纳米金(HGNs)的生物传感器检测双酚A(BPA)。空壳纳米金通过透射电子显微镜(TEM)进行了表征。采用循环伏安法和电化学阻抗等方法研究修饰电极的电化学响应特性。由于石墨烯和空壳纳米金良好的生物导电性和生物兼容性,该传感器对双酚A有较好的电流响应。双酚A浓度在1.0×10-7mol/L到7.85×10-6mol/L的范围内与传感器响应电流呈线性关系,检测限为5.2×10-8mol/L(S/N=3)。该生物传感器证明有很好的重现性、稳定性和抗干扰能力。

A novel multilayer biosensor based on prussian blue （PB）, graphene, tyrosinase （Tyr） and hollow gold nanoparticles （HGNs） for the determination of bisphenol A （BPA） was prepared. The HGNs were characterized by transmission electron microscopy （TEM）. Cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical properties of the modified electrode. The sensor exhibited good amperometric response towards BPA due to the excellent biological conductivity and biocompatibility of graphene and HGNs. The method showed good linearly for 1.0×10^-7～7.85×10^-6 mol/L BPA with a detection limit of 5.2×10^-8 mol/L （S/N=3） under the optimal conditions. The biosensor showed good performance in reproducibility, stability and interference experiments.