茶树品种资源遗传多态性RAPD分析

Study on diversity of tea germplasm by RAPD method

探讨了采用RAPD分子标记法进行茶树及其近缘种分类和亲缘关系鉴别的可能性。结果表明 ,RAPD能有效地区分物种间和茶树种内变种间的差异 ,阿萨姆类型茶树和中国类型茶树的特异性RAPD分子标记分别为 1 2 0 0bp的WKA 2 4a和 61 0bp的WKA 2 4b。日本茶树品种与中国茶树品种具有较近的亲缘关系。部分越南茶树品种为中 印杂种。图 1表 1参

An RAPD method was used to investigate the possibility of classification and consanquinity identification of Camellia sinensis and its close consanquineous species.The results showed that species and variety specificity could be identified by RAPD method.Camellia sinensis var. assamica and C.sinensis had the specific band WKA 24a with 1 200 bp and band WKA 24b with 610bp,respectively.Japanese tea varieties were more closeto Chinese tea varieties.Part of tea varieties from Vietnam was hybrid of Camellia sinensis var. assamica and C.sinensis.