IBV地方分离株致病原性和血清学分型的比较

PATHOGENICITY AND SEROTYPE OF AVIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES FROM CHINA

将 6株分离自国内不同地方的 IBV流行株分别感染 SPF鸡 ,对气管和肾脏进行系统病理观察 ,发现 6株 IBV在致病原性和组织嗜性上存在显著差异 ,其中 QD可引起严重的呼吸道损伤 ,并伴有相对较轻的肾脏损伤 ,其余 5株对肾脏损伤较为严重而对呼吸道损伤相对温和。用气管环组织培养交叉中和实验 (SN)和 SAS软件对 6株分离毒进行血清学分型研究 ,结果显示 QD株属于 M型 ,ZZ、GZ属于 T型 ,而 TJ、DL和 YC株是不同于 M和 T血清型的变异株。研究结果表明 ,不同 IBV毒株的致病原性和血清型有一定的相关性 ,同时也说明我国 IBV流行株已发生变异 ,并存在所谓肾型

SPF chickens were challenged with 6 IBV isolates from different regions in China, different predispositions of tissue tropism occurred ,and histopathologic oberservation was made among the 6 isolates.QD caused more severe lesions in trachea and milder lesions in the kidney,while others caused more severe renal lesions and mild tracheal lesions.Compared with 3 reference strains M 41 ,H 52 and T, and by using cross neutralisation tests(SN) of antiserums and SAS computer program,it was showed that QD strain belonged to M 41 serotype ,and ZZ,GZ belonged toT serotype ,meanwhile the others were novel variants which differ from M or T serotype.These results indicate that the pathogenicity of different IBV strains is relative to their serotype,while the IBV isolates in China are variant strains,and the epidemic nephropathogenic IB is prevailing in China.