早期使用三七总皂苷对大鼠脑出血炎性反应的影响

The Effect of Early Use of Panax Notoginseng Saponins on Inflammation in Rats with Cerebral Hemorrhage

目的:早期使用三七总皂苷对脑出血大鼠(Intracerebral Hemorrhage,ICH)脑组织病理形态和炎性反应递质细胞间黏附分子-1(Intercellular Adhesion Molecule-1,ICAM-1)和肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)的影响。方法:将SD大鼠随机分为假手术组、模型组和三七总皂苷组,采用胶原酶注射尾状核法制作脑出血模型,观察脑组织病理形态变化;双抗体夹心-酶联免疫吸附实验(Avidinbiotin complex-ELISA,ABC-ELISA)观察ICH大鼠血清炎性反应递质ICAM-1和TNF-α的变化。结果:HE染色观察到炎性细胞浸润,也证实了脑出血急性期炎性反应反应较剧烈,三七总皂苷显著减轻周围脑组织水肿和神经纤维排列紊乱现象;造模7d时,模型组血清中的ICAM-1和TNF-α含量增加,三七总皂苷用药7d可下调ICAM-1(P<0.05)和TNF-α。结论:三七总皂苷对脑出血后脑组织病理形态恢复正常具有促进作用,抑制ICAM-1和TNF-α的释放,可能是神经元保护的作用机制之一。

Objective： To study the effect of early use of Panax notoginseng saponins （ PNS） on the pathomorphological brain tisssues, ICAM-1and TNF-α in rats with cerebral hemorrhage（ ICH） . Methods： SD rats were randomly divided into sham group,model group and PNS group,and ICH models were made with collagenase Ⅶ injected into the rats’caudate nucleus. The morphological changes in the brain were observed. ICAM-1 and TNF-α in the serum were measured by the method of enzyme linked immunosorbent assay （ ELISA） . Results： The region around the hematoma infiltrated by inflammatory cells was observed by HS staining,and PNS significantly reduced the brain edema andameliorated disorderly arrangement of nerve fibers; On the 7th day after ICH,the level of ICAM-1 and TNF-α in the serum was still higher in the model group compared with that in the sham group,and the administration of Panax notogin-seng saponins down-regulated ICAM-1（ P0. 05） and TNF-α. Conclusion： Panax notoginseng saponins can alleviate the pathomorpho-logical brain tissues of cerebral hemorrhage,and it could inhibit the release of ICAM-1 and TNF- α so as to protect the neurons.