摘要
以专利菌株紫色红曲霉(Monascus purpure)MPT13为供试菌株(专利号:201010182965.9),通过液态发酵试验优化其培养条件,研究其产洛伐他汀的最佳工艺条件。结果表明,MPT13菌株的最优液体发酵培养条件为:以甘油作为最佳碳源、大豆粉作为最佳氮源,最佳培养基成分(g/L)为:甘油90 mL,大豆粉7.5 g,MgSO40.5 g,NaNO32 g,KH2PO41.5 g,水500 mL,培养温度28℃,装液量20%,接种量5%,培养12 d。
The fermentation conditions of Monascus purpure (MPT13) were optimized for the production of lovastatin by liquid fermentation experiments. The results showed that glycerol is the best carbon source and soybean flour is the best nitrogen. The optimum liquid fermentation conditions of MPT 13 were as follows: the optimal medium composition (g/L) was glycerol 90 mL, soybean flour 7.5 g, MgSO4 0.5 g, NaNO3 2 g, KH2PO4 1.5 g, H20 500 mL; the temperature is 28℃; the volume of inoculums was 5% of the volume of fermentation medium, the volume of the medium in the fermentation flask was 20% of the volume of the flask; cultivating period was 12 d.
出处
《食品工业》
北大核心
2013年第8期70-72,共3页
The Food Industry
基金
国家自然科学基金项目(31260197)
云南省自然科学重点基金项目(2009CC005)
国家科技支撑计划项目课题(2007BAD58B02)
云南省科技厅富民强县计划项目(2011EB117)