RP-HPLC法测定酚酞片中酚酞的含量

Content determination of Phenolphthalein tablets by RP-HPLC

目的建立RP-HPLC法测定酚酞片的含量。方法采用Agilent C18色谱柱(5μm,4.6 mm×150 mm),柱温为25℃,以甲醇-0.05 mol/L NH4H2PO4(用磷酸调pH至3.31)(70:30)为流动相,流速为1 mL/min,检测波长为275 nm。结果酚酞在(19.968～139.776)μg/mL的范围内线性关系良好(r=0.9999,n=5),测得平均回收率为100.87%,RSD=0.88%(n=9)。结论该方法稳定可靠,重现性好,可有效控制酚酞片的质量。

Objective To develop an HPLC method for the determination of the content of Phenolphthalein tablets. Methods Agilent Czs column（5 μm, 4.6 mm×150 mm）was used, the column temperature was 25℃, the mobile phase was methanol -0.05 mol/LNH4H2PO4 （adjusted to pH 3.31 with phosphoric acid） （70：30） at the flow rate of 1.0 ml min-1, The UV detection wavelength was 275 nm. Results The linear range was （19.968-139.776） μg/mL （r = 0.9999,n = 5）, the average recoveries was 100.87% with RSD = 0.88% （n = 9）. Condusion This method is stable, reliable and repro- ducible. It can be used for the aualitv control of Phenolnhthalein tablets.