肺血栓栓塞症患者血小板Rho GTP酶相关mRNA差异性表达特征

The significance of the differential expression of Rho GTPases mRNA of platelet in pulmonary thromboembolism patients

目的检测分析肺血栓栓塞症(PTE)患者血小板Rho GTP酶相关mRNA表达水平,分析其差异表达的意义。方法随机取选20例PTE患者为PTE组;20例年龄、性别与之匹配的非PTE患者为对照组,提取外周血单个核细胞总RNA,采用人类全基因组表达谱芯片比较两组PTE患者血小板Rho GTP酶mRNA表达的差异,并进行FQ-PCR验证芯片的可靠性。结果检测出血小板Rho GTP酶相关基因mRNA 11条,两组比较,PTE组mRNA表达有3条(Rho GTP酶Rac1蛋白及其鸟嘌呤核苷酸交换因子Vav1、Vav3)高于对照组,差异具有高度统计学意义(P<0.01)。结论 PTE组中部分血小板Rho GTP酶相关基因mRNA表达显著上调,提示血小板功能部分活化。

Objective To detect the expression of Rho GTPases of platelet in pulmonary thromboembolism, to analyze the significance of its differential expression. Methods Twenty patients with PTE were enrolled in our study and the other twenty patients without PTE were allocated as controls. Total RNA was extracted from isolated mononuclear cells in peripheral blood samples of twenty patients and controls. Significant differential mRNA expression of Rho GTPases were analyzed by means of mRNA expression microarray. Results Eleven pieces of mRNA fragments associated with Rho GTPases were screened and part of the genes were up-regulated in PTE group compared with control group （P 〈 0.05）. The up-regulated mRNA fragments associated with Rho GTPases were Rho GTPase Racl ,Rho GEF Vavl and Vav3（P 〈 0.01 ）. Conclusion Part of Rho GTPases mRNA of platelet were up-regulated in PTE ,platelet functions maybe partially activated.