苦豆子植物的薄层色谱鉴别

Thin-Layer Chromatography Identification of Sophora Alopecuroides

目的:为鉴别苦豆子植物主要生物碱成分及提取物制定其相关的质量标准提供参考依据,以便进行质量控制。方法:对苦豆子果实及其提取物、苦豆子草及其提取物利用薄层色谱法进行二次展开,并与苦豆子总碱及苦参总碱进行对比。结果:所建立的薄层色谱条件能有效分离单体斑点。结论:薄层色谱二次展开斑点清晰,方法简便、快捷、准确、重复性好对苦豆子植物质量及性质鉴别有指导意义。

Objective ： To provide the reference for the identification of components and extract of Sophora alopecuroides for establishing the quality standards, in order to carry out the quality control. Methods：Sophora alopecuroides fruit and its extract, Sophora grass and its extracts were for the second expansion with thin -layer chromatography. The produc- tions were compared with productions of Aloperinan alkaloid and total matrine. Results ： The plants and extracts of the al- kaloids and standard spots were consistent. Conclusion ： The TLC spots were clear for the second expansion. The method is simple, fast, accurate, and has good repeatability of Sophora alopecuroides L. plant quality identification.