解毒活血方对糖尿病足常见感染细菌作用研究

Study on Sensitivity of Jiedu Huoxue Prescription to Common Infected Bacteria of Diabetes Foot

目的:研究临床常用的解毒活血方(组成:黄连、黄芩、黄柏、当归、川芎、苦参)对糖尿病足常见感染细菌的作用,探讨其临床作用机理。方法:解毒活血方分别采用颗粒剂型和生药饮片两种剂型,采用琼脂稀释法,测定解毒活血方两种剂型的最低抑菌浓度(minimum inhibitory concentration,mic)。结果:解毒活血方对糖尿病足常见的细菌均有不同程度的抑菌作用,颗粒剂型效果略优于生药饮片剂型,两种剂型均对金黄色葡萄球菌的抑菌效果较佳,在药物浓度为31.25 mg/mL时,颗粒剂型对金黄色葡萄球菌的抑菌率即达100%,在药物浓度为62.50mg/mL时,生药饮片剂型对金黄色葡萄球菌的抑菌率达85%,对大肠杆菌抑菌效果稍差。结论:解毒活血方对糖尿病足常见的细菌均有抑菌作用,其对细菌的抑制作用可能为其临床治疗糖尿病足的作用机理之一。

Objective：Antibacterial property of commonly used Jiedu Huoxue Prescription （Composed of optis Chinch- sis, Scutelaria Baicalensis, Phellodendron Chinensis, Angelica Sinensis, Ligusticum Chuanxiong, Sophora Flavescens） to common infected bacteria of diabetes foot, to explore its clinical mechanism of action. Method ： Selecting particle formu- lation and crude drugs and using AGAR dilution method, determination of minimum inhibitory concentration of two formuations was made. Results ： Jiedu Httoxue Prescription had different bacteriostasis functions to common bacteria of diabetes foot, particle formulation was slightly better than crude drugs. Both had better antibacterial effect on Staphylococcus au- reus and less effective on E. coli. When the drug concentration was 31.25 mg/mL, particle formulation inhibitory rate of Staphylococcus aureus was 100% , and when the drug concentration was 62.50 mg/mL, the crude drug formulation inhibitory rate was up to 85%. Conclusion ：Jiedu Huoxue Prescription has bacteriostasis function to common infected bacteria of diabetes foot, inhibition of bacteria may be its one of the clinical treatment mechanisms of diabetic foot.