原发性胆汁性肝硬化患者血清AMA-M2及其靶抗原检测的阳性率对比

Contrast of AMA-M2 and its Target Antigen Detection Sensitivity in Patient's Serum with Primary Biliary Cirrhosis

目的:针对PBC患者血清同时选用国产和进口两种试剂盒检测AMA-M2及其靶抗原:丙酮酸脱氢酶复合体E2亚单位(PDC-E2)及抗M2-3E等免疫指标,并对其敏感性进行对比,观察两种试剂盒检测有无差异及3个指标之间的敏感性。方法:对51例PBC确诊患者的血清标本,采用上海丰翔生物公司及欧蒙公司的ELISA试剂盒对待测血清分别进行了AMA-M2、抗M2-3E、PDC-E2等指标的检测,进行阳性率的比较。结果:51例患者血清标本中,国产试剂盒检测AMA-M2、抗M2-3E、PDC-E2的阳性率分别为72.5%、90.2%、72.5%,进口试剂盒检测AMA-M2、抗M2-3E、PDC-E2的阳性率分别为78.4%、92.2%、82.4%,经统计软件处理得到的结果P>0.05,无明显的统计学差异。针对42例AMA阳性的患者进行AMA-M2、抗M2-3E、PDC-E2检测时,国产试剂盒检测的阳性率分别为85.7%、95.2%和78.6%,进口试剂盒检测的阳性率分别为95.2%、100%、95.2%,经统计软件处理后得出P>0.05,两种试剂盒检测无明显的差别。结论:使用进口和国产两种试剂盒检测AMA-M2、PDC-E2、抗M2-3E的阳性率无明显的统计学差异。AMA-M2、PDC-E2、抗M2-3E 3个指标中阳性率最高的是抗M2-3E,AMA-M2和PDC-E2差异无统计学意义。

Objective：This experiment chose domestic and imported ELISA kits to test AMA-M2 and its target antigens： E2 subunits of the pyruvate dehydrogenase complex and anti-M2-3E etc in serum of patients with PBC,to compare the sensitivity between two kits,and observed the sensitivities of three indexes by using two kinds of kit.Methods：We detected the AMA-M2,anti-M2-3E and PDC-E2 indexes for 51 patients with PBC by FengXiang biotechnology company in Shanghai and EUROIMMUN Mikrotiter-ELISA kits,to compare the positive rate after statistics treatment.Results：51 serum samples of patients with PBC,the positive frequencies were 72.5% for AMA-M2,90.2% for anti-M2-3E,72.5% for PDC-E2 by domestic biological detection kit,and 78.4% for AMA-M2,92.2% for anti-M2-3E,82.4% for PDC-E2 by import kit.42 serum samples of patients with positive AMA,the positive frequencies of AMA-M2,anti-M2-3E and PDC-E2 were 85.7%,95.2% and 78.6% respectively by domestic biological kit,95.2%,100% and 95.2% respectively by import kit,There was no obvious difference in detection results between two kits after statistical processing（P0.05）.Conclusion： The positive frequencies of AMA-M2,PDC-E2,anti-M2-3E by import kit were higher than domestic kit from distribution,but the results of statistical processing（P0.05） were no obvious difference.No matter that the test kit,the positive frequency of anti-M2-3E was the highest then AMA-M2 and PDC-E2.The positive frequencies of AMA-M2 and PDC-E2 were no obvious difference.