猪伪狂犬病毒的分离与鉴定

Identification of a Swine Pseudorabies Virus Field Isolate

从河南某发病猪场大批死亡仔猪的脾、肾及脑组织中分离到1株猪伪狂犬病毒(PRV)毒株,该毒株经细胞连续传代培养后,接种PK-15细胞能够产生明显的细胞病变(CPE),且能被PRV阳性血清中和。病毒对氯仿、乙醚敏感,56℃水浴30min能使其灭活。将所分离的病毒接种家兔和小鼠,均出现明显的伪狂犬病临床症状。PCR鉴定结果表明,用gp50基因序列引物能够从该毒株上扩增出目的条带,将测序结果与GenBank中的5株PRV毒株进行比对分析,同源性达99%。证实所分离病毒为猪伪狂犬病毒。

An isolate of pseudorabies virus（PRV） was obtained from the tissues of dead piglets from a farm with PRV-like disease in Henan province. This isolate of PRV could induce typical cytopathogenic effects after several passages on PK-15 cells and be neutralized by PRV positive serum in microneutralization test. This isolate was sensitive to chloroform and ether,and could be inactivated in 56 ℃ water bath after 30 min. After inoculation,both rabbits and mice showed typi- cal clinical symptoms of pseudorabies. The cloning and sequencing of gp50 gene showed that the nucleotide homology of this isolate with other 5 PRV isolates previously published in GenBank was 99% ,indicating that this isolate is PRV.