草鱼内脏复合蛋白酶提取工艺研究

Study on Extraction Process of Compound Protease from Viscera of Grass Carp

为充分利用草鱼加工下脚料,以草鱼内脏为原料,以复合蛋白酶酶比活为指标,对草鱼内脏复合蛋白酶的提取、分离、干燥条件进行优化。结果表明,最佳的草鱼内脏脱脂物是工业己烷;最佳的复合蛋白酶提取条件为磷酸盐缓冲液pH值8、提取温度20℃、浸提时间80min,超声波辅助提取频率15kHz、功率50W、时间4min;最佳的复合蛋白酶分离方法是添加质量浓度为8g/L的茶多酚;最佳的干燥条件是聚乙二醇终质量浓度20g/L、装液厚度6mm、快速冷冻、干燥时间24h。在此条件下,复合蛋白酶酶比活为4.33U/mg。

In order to make full use of scraps of grass carp processing, the extraction,separation, and drying process of compound protease from viscera of grass carp were optimized with specific activity of compound protease as an index. The results showed that the best extraction condition of compound protease was pH value of 8, extraction temperature of 20 ＊C, extraction time of 80 min,ultrasonic frequency of 15 kHz, ultrasonic power of 50 W, sonification time of 4 min; the best separation condition of compound protease was tea polyphenol of 8 g/L; the best drying condition of compound protease was polyethylene glycol of 20 g/L, material thickness of 6 ram, quick-freezing, drying time of 24 h. Under the conditions,the specific activity of compound protease was 4.33 U/rag.