发光细菌法检测饮用水综合毒性的应用研究

Study on detection of complex toxicity of drinking water by luminescent bacteria

目的应用发光细菌法,选用81.9%低毒性测试模式,分析饮用水的综合毒性。方法通过各种模拟实验,建立生活饮用水的模拟生物毒理性基线以及分析对发光菌试验的影响因素,说明在饮用水应急性毒性检测中的应用价值。结果建立某地区水质生物毒理性基线为-11.0%～18.4%,作为水质综合毒性判断依据;发光菌发光影响因素实验表明,pH值、色度、浑浊度、余氯等因素对发光菌发光有抑制作用,需要在测试之前对水样进行预处理。结论通过建立生物毒理性基线,发光细菌法可以快速地判定水样的毒性强弱,可作为监测水质突发性污染事故及水质突变的应急方法。

[ Objective ] By using the luminescent bacteria method, a simulation experiment was carried out with 81.9% low toxicity model, to analyze the complex toxicity of drinking water. [ Methods ] By simulation experiments, the biological toxicological base- line of drinking water was established, and factors that influenced the luminescent bacteria test were studied, to explain the applica- tion value of this method in emergency toxicity detection of drinking water. [ Results] The biological toxicological baseline of water quality in a region was - 11.0% - 18.4%, which can be used to detect the comprehensive toxicity of drinking water. The factors, including pH value, chrominance, turbidity and residual chlorine, would inhibit the luminosity of luminescent bacteria, so the wa- ter samples would be pretreated before test. [ Conclusion] Based on the biological toxicological baseline, the luminescent bacteria method can rapidly determine the toxicity of the drinking water, which can be used as the emergent method for monitoring the sud- den water pollution accident and water quality mutation.