摘要
目的建立生新发胶囊(补骨脂、何首乌、女贞子、当归、天麻、黄精等)的指纹图谱,更好地控制生新发胶囊的质量。方法采用HPLC法,二极管阵列检测器(PADA);流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;柱温35℃;体积流量为1.0 mL/min;检测波长246 nm。结果 HPLC-PADA色谱图中有13个共有峰,采用国家药典委员会"中药色谱指纹图谱相似度评价系统(2004A版)"自动处理,11批生新发胶囊的相似度均大于0.99。结论该方法准确可靠、简便、稳定,重复性好,可作为生新发胶囊的质量评价方法。
Objective To establish the fingerprint of Shengxinfa Capsules（consisted of Psoraleae Fructus,Polygonui Multiflori Radix,Ligustri Lucidi Fructus,Angelicae Sinensis Radix,Gastrodiae Rhizoma,Polygonati Rhizoma,etc.） for the quality evaluation.Methods The fingerprint was established by HPLC with Photodiode Array Detector（PADA）.The acetonitrile-0.1% phosphoric acid（gradient elution） was employed as the mobile phase,column temperature was at 35 ℃,flow rate was 1.0 mL/min,and detection wavelength was at 246 nm.Results There were 13 common peaks appeared in the PADA fingerprint,which were defined by software published by the Chinese Pharmacopeia Committee.The similarity of 11 batches was more than 0.99.Conclusion The method is simple,accurate,and sensitive.It is suitable to be used for the quality evaluation of Shengxinfa Capsules.
出处
《中草药》
CAS
CSCD
北大核心
2013年第14期1935-1938,共4页
Chinese Traditional and Herbal Drugs
基金
广西壮族自治区科技公关项目(桂科攻1099062-11)
