接骨木根皮促进骨折愈合作用的研究

Promotion of Sambucus williamsii root barks on fracture healing

目的观察接骨木根皮促进骨折愈合的作用。方法制备桡骨骨折家兔模型。实验分对照组,模型组,跌打丸(0.28g/kg)阳性对照组,接骨木低、中、高(0.17、0.34、0.68 g/kg)组,药物掺入饲料,于造模后第2天连续给药36 d。观察接骨木根皮对骨折区骨组织形态和血清相关生化指标的影响。结果与模型组比较,给药12 d,即骨折愈合初期,接骨木高、中剂量组兔血清碱性磷酸酶(ALP)、酸性磷酸酶(ACP)水平显著升高(P<0.05、0.01);给药24 d,即骨折愈合中期,接骨木高、中剂量组兔血清骨钙素(BGP)水平显著升高(P<0.05、0.01)。X光片显示,接骨木中剂量组兔骨膜反应密度较深,骨痂增深、量增多,且其密度与骨密度相似;光镜观察显示骨膜下新生骨组织形成均质的骨小梁,新生骨组织对原骨质两端成"工"字形包裹。结论接骨木根皮通过促进成骨细胞合成、分泌ALP和BGP及破骨细胞分泌ACP,提高成骨细胞的数量、加快局部坏死骨质的吸收及骨基质的成熟和钙化,从而加快骨折愈合。

Objective To observe the promotion of Sambucus williamsii（SW） root barks for fracture healing.Methods Rabbit models with radius fracture were established.The rabbits were divided into control,model,Dieda Pill negative control,and low-,mid-,and high-dose（0.17,0.34,and 0.68 g/kg） of SW groups.Drugs were added into forage and were given to rabbits 2 d after modeling for 36 d.The effect of SW root barks on serum biochemical indexes and histomorphology of fracture healing was observed.Results Compared with the model group,a significant increase of alkaline phosphatase（ALP） and acid phosphatase（ACP） was observed in high-and mid-dose SW groups in the initial stage of fracture healing of 12 d after administration（P 0.05,0.01）.A significent increase of serum bone gla protein（BGP） was observed in the high-and mid-dose SW groups in the middle stage of fracture healing of 24 d after administration（P 0.05,0.01）.X-ray revealed that deep periosteal reaction density was found,osteotylus was higher and more in the mid-dose SW group,and the density was similar to bone density;Light microscope revealed that trabecular bone appeared in new born bone tissue,and the substance of bone was encapsulated with I-section new born bone tissue.Conclusion SW root barks have the active fraction for fracture healing by promoting the synthesis and secretion of ALP and BGP of osteoblast,promoting the synthesis of ACP in osteoclast,increasing the quantity of osteoblasts,promoting the absorption of necrotic bone,and speeding up the maturation calcification of bone matrix.