天津市MSM HIV感染窗口期检测策略的研究

A study on early detection of HIV infection among MSM in Tianjin

目的探索适用于男男性行为人群(MSM)的艾滋病病毒(HIV)早期感染筛查策略,并对利用早期感染数据估测新发感染率的可行性进行评价。方法常规HIV抗体检测结合集合核酸检测,样品集合及分拆方法按50∶5∶1进行。结果 5 287份MSM血样中,5 073份HIV抗体检测阴性,5份样品为HIV抗体不确定,209份HIV抗体阳性。经核酸检测,5 073份HIV抗体阴性样品中,检出10份阳性;5份HIV抗体不确定样品均为阳性。15份核酸阳性样品中,有8例进行随访检测,均出现HIV抗体阳转。结论常规HIV抗体检测结合集合核酸检测可以发现更多的感染者,降低检测成本,对MSM中HIV感染窗口期诊断和HIV发病率的估测有重要意义。

Objeetive To explore the early infection screening strategy for men who have sex with men （MSM） population and evaluate the feasibility of new infection rate and of early infection data estimate. Methods Human immunodeficiency virus （HIV） antibody test combined with a pooled nucleic acid testing. Sample collection and the spin-off press was 50 = 5 ： 1. Results Of the 5 287 blood samples collected from MSM, 5 073 were HIV antibody negative, five samples for HIV antibody were uncertain, 209 were HIV antibody positive. Ten out of 5 073 HIV an- tibody negative samples were tested nucleic acid-positive; five HIV antibody uncertain samples were positive by nu- cleic acid testing; eight out of 15 nucleic acid-positive samples were HIV antibody seroeonversion in follow-up test- ing. Conclusion Conventional HIV antibody test combined with pooled nucleic acid testing can detect more infec- tion, therefore to reduce testing cost, and is helpful to HIV window period diagnosis and HIV incidence estimates a- mong MSM.