移植自体嗅黏膜对脊髓损伤大鼠脊髓内MMP-2、9表达的影响

Effects of Autologous Transplantation of Olfactory Mucosa on Expression of MMP-2 and MMP-9 in Injured Rat Spinal Cord

目的探讨自体嗅黏膜移植干预脊髓损伤(spinal cord injury,SCI)大鼠脊髓组织内基质金属蛋白酶-2、9(MMP-2、9)的表达与动物神经功能恢复的关系。方法制作SD大鼠半横断脊髓损伤模型,按随机数字表法分为模型对照组和自体嗅黏膜组,每组20只(同时取5只大鼠设为正常对照组)。自体嗅黏膜组在损伤处植入自体嗅黏膜,移植后1、3、7、14d(每时间点5只大鼠)采用BBB评分评价神经功能、免疫组织化学法检测脊髓内MMP-2、9表达。结果 SCI后自体嗅黏膜组BBB评分高于模型对照组(P<0.05)。各时间点SCI大鼠脊髓内MMP-2、9的表达显著升高(正常对照组大鼠未见MMP-2、9的表达)。伤后同一时间点自体嗅黏膜组脊髓内的阳性细胞数少于模型对照组(P<0.05)。结论自体嗅黏膜移植可明显促进神经运动功能恢复情况;此种作用可能与其能够抑制MMP-2、9过度表达有关。

Objective To study the relationship between the expression of MMP-2 and MMP-9 and the recovery of neurological function in rats undergoing autologous transplantation of olfactory mucosa for injured spinal cord.Methods Healthy Sprague-Dawley rats with spinal hemisection were randomly divided into two groups：control group and autologous olfactory mucosa transplantation group,with 20 rats in each group（Five rats were set to the mormal control group）.Neurological function was evaluated by the Basso-Beattie-Bresnahan（BBB） scale and MMP-2 and MMP-9 expression in injured spinal cord was detected by immunohistochemistry 1,3,7 and 14 days after transplantation（5 rats per time point）.Results After spinal cord injury,the expression of MMP-2 and MMP-9 obviously increased at all time points（Normal control group no MMP-2 and MMP-9 expression）.Compared with control group,the BBB scores increased but the number of MMP-9 and MMP-2 positive cells decreased at the same time point in autologous olfactory mucosa transplantation group（P〈0.05）.Conclusion Autologous olfactory mucosa transplantation can promote the recovery of neurological function through inhibiting the expression MMP-9 and MMP-2 in injured rat spinal cord.