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乳制品中乳铁蛋白的测定 被引量:10

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摘要 采用高效液相色谱法建立了乳制品中乳铁蛋白测定的分析方法。试样中的乳铁蛋白经磷酸盐缓冲液提取,提取液经肝素亲和柱净化后,采用反相高效液相色谱柱分离,以二极管阵列检测器于280nm处检测,采用外标法定量。结果表明:乳铁蛋白在10.0~1000.0ug/mL范围内线性良好,添加回收率为75%~110%,相对标准偏差(RSD)小于20%。该方法具有灵敏度高、准确度高、重现性好的特点,适合检测机构或乳品企业日常样品中乳铁蛋白的检测。
出处 《现代农业科技》 2013年第16期291-292,共2页 Modern Agricultural Science and Technology
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参考文献7

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二级参考文献53

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