摘要
建立一种经济、方便测定草铵膦铵盐的高效液相色谱检测方法。在碱性条件下草铵膦与氯甲酸-9-芴甲酯(FMOC-Cl)反应,对衍生物进行液相色谱分离和测定。以甲醇+乙腈+水=25+25+50为流动相,C18为分离柱,在波长265nm下检测。本法衍生反应快,衍生产物稳定,铵离子不影响,在0.010 18-0.203 6mg/mL范围内线性关系良好(r=0.999 9)。方法的标准偏差为0.555,变异系数为0.768%,添加回收率98.9%-100.6%,检测限为0.4μg/mL。本方法使用C18柱较耐用,检测灵敏度高,适合于草铵膦原药及制剂的测定。
To establish a convenient HPLC method for the determinnation of Glufosinate-ammonium.After derivatization with 9-fluorenylmethylchloroformate（FMOC-Cl）,the derivative was quantified by RP-HPLC on a C18 column with the mobile phase of methanol-Acetonitrile-water（25 ＋25 ＋50） and detection at 265nm.The results showed that the derivative was stable and rapidly achieved,and has no disturb by NH4＋.The linear calibration was found with in the range of 0.010 18 ～0.203 6mg/mL（r =0.999 9）.The relative standard deviation and the correlation coefficients was 0.555 and 0.768% respectively.The recovery ranged from 98.9% to 100.6% and the detection limit was 0.4μg/mL.The method is suitable to the determination of Glufosinate-ammonium and its formulation with durable column and high sensitivity of detection.
出处
《农药科学与管理》
CAS
2013年第8期51-54,共4页
Pesticide Science and Administration
