p53和p27在肺癌组织中的表达及其临床意义

The expresssion of p53 and p27 in lung cancer and its clinical significance

目的检测肺癌组织中p53和p27蛋白的表达情况，以探讨其相关的临床意义。方法选择2011年12月至2012年12月收治的肺癌患者组织标本100例，另选取同期肺部良性病变切除的正常肺组织40例作为正常对照。采用免疫组织化学法检测p53和p27蛋白的表达。结果p53蛋白在正常肺组织中的阳性表达率为27．5％（11／40），显著低于肺癌组织中的66．0％（66／100），差异有统计学意义（P〈0．05），而p27蛋白在正常肺组织中的阳性表达率为87．5％（35／40），显著高于肺癌组织中的41．0％（411100），差异有统计学意义（P〈0．05）。肺癌组织中p53和p27蛋白的表达与患者的性别、肿瘤组织类型无显著相关性（P〉0．05），而在不同临床分期存在显著相关性（．P〈0．05）。Spearman相关性分析显示肺癌组织中p53和p27蛋白的表达呈显著负相关（r=-0．516，P〈0．05）。结论p53和p27蛋白的异常表达可能参与了肺癌的发生、发展过程，二者的联合检测有可能成为肺癌诊断以及预后评估的新分子标记物。

Objective To investigate the expression and clinical significance of p53 and p27 in lung cancer. Methods From December 2011 to December 2012,100 cases of patients with lung cancer wereenrolled in this study. Another 40 person without lung cancer were collected as normal control. The protein expression of p53 and p27 was detected by immunohistochemistry. Results The positive rate of p53 in normal control was lower than that in lung cancer patients [27.5%（11/40） vs. 66.0%（66/100） ]（P〈 0.05）. The positive rate of p27 in normal control group was higher than that in lung cancer group [ 87.5% （35/40） vs. 41.0% （41/100） ] （P 〈 0.05 ）. The expression of p53 and p27 had no relationship with the gander and tumor tissue type in lung cancer patients （P 〉 0.05）, but had relationship with the clinical pathologic stage （P 〈 0.05）. Spearman correlation analysis showed that the expression of p53 and p27 protein in lung cancer tissue had significantly negative correlation （r = -0.516, P 〈 0.05 ）. Conclusion The abnormal expression of p53 and p27 may be involved in the occurrence and development of lung cancer, and their joint detection may be used as the new molecular marker in the diagnosis and prognosis assessment of lung cancer.