Bak基因对Hela细胞的细胞周期和细胞凋亡的调控作用(英文)

Effect of Bak on regulation of cell cycle and apoptosis in Hela cells

目的研究Bcl 2基因家族促凋亡蛋白Bak在细胞凋亡细胞周期调控中的作用 ,评价它们作为肿瘤治疗的潜在靶基因的可能性。方法采用一种可诱导性表达系统,(MT II调节系统),在外加锌离子(ZnSO4,100μmol/L)的条件下诱导Bak基因表达。以Hela细胞系作为靶细胞 ,获得表达Bak基因的稳定转染子。结果可诱导性Bak基因过表达的Hela细胞出现广泛死亡。TUNEL染色证实细胞核碎片化 ,提示细胞死亡是凋亡。流式细胞仪显示 ,在诱导后24h有19.29 %的细胞发生凋亡且细胞在G1 期明显积聚。结论Bak基因能显著诱导Hela细胞凋亡和细胞周期在G1 期延长。因此 。

Aim We are currently investigating the role of Bcl 2 related pro apoptotic protein Bak in apoptotic and cell cycle pathways, and are evaluating its potential as a target gene for therapeutic intervention. Methods We used an inducible expression system, MT II regulatory system, which allowed controlled expression of protein upon addition of ZnSO4(100 μmol/L) as an external inducer. Rapidly growing Hela cell line was chosen as a target. Stable transfecting inducible expression vector containing Bak gene was performed. Results Cells overexpressing Bak showed extensive cell death with nucleus fragmentation detected by TUNEL assay. FACS analyses showed Bak induced apoptosis in 19.29 %cells 24h after induction and a significant accumulation in G1 phase was observed. Conclusion This vector system enables us to evaluate the anti tumor effect of Bak in vitro; Bak may prolong cell cycle in G1 phase and lead to apoptosis. Therefore, Bak gene may be a good candidate for cancer gene therapy.