rDNA-ITS序列分析鉴定块菌伴生真菌

rDNA-ITS Sequence Analysis Identify Truffle-associated Fungi

分离攀枝花地区野生块菌伴生真菌,经PCR扩增其rDNA-ITS序列,测序并分析其序列信息,初步鉴定了7种块菌伴生真菌.系统进化树分析将供试真菌分为3大类群:植物腐生真菌、动物病原真菌及植物内共生真菌.

Wild truffle-associated fungi in Panzhihua area were separated and their rDNA-ITS sequences were amplified by PCR.The sequencing and analysis of ITS sequences preliminarily identified seven fungi species associated with truffle.Phylogenetic analysis results shown that these fungi can be divided into 3 groups： plant saprophytic fungi,animal pathogenic fungi and plant symbiotic fungi.