三联法制备猕猴桃果肉中果胶的工艺研究

Extraction of Pectin from Actinidia Pomace by Triple Technology in Box-Behnken Model

分别研究了盐酸-微波法和纤维素酶(Cellulase,Case)-微波法制备猕猴桃果肉中可溶性膳食纤维-果胶(SDF-P),在此基础上,用Box-Behnken响应曲面优化酶解程序,进行三联工艺的整合,试图有效提高SDF-P得率和含量.结果表明,经0.2%盐酸预处理(500 W/40 s微波)后的果肉粉,进入酶解环节并优化,得到的最佳酶解工艺条件为体积浓度0.2%Case、15∶1(V∶W)、500 W和30 s微波处理.采用优化后的三联工艺,极大提高猕猴桃果肉中SDF-P含量至(48.8±0.03)%(p<0.01),纯度提高2.8倍;同时其得率也达到11%左右,分别是酸解或酶解工艺的1.7倍和2.1倍.由此可见,采用酸-酶-微波的三联工艺,可高效制备猕猴桃果肉SDF-P,降低工业成本.

This paper studies the preparation of soluble dietary fiber-pectin（SDF-P） from actinidia pomace, in which HC1- microwave is contained, and cellulase-microwave treatments respectively. On this basis, eellulase hydrolysis procedures are optimized by the Box-Behnken response surface for effectively increasing the yield and content of SDF-P. The results show that actinidia pomace with O. 2% HC1 （ 500 W/40 s microwave ） initially comes into eellulase hydrolysis. The optimal conditions for extraction are achieved by treating O. 2% cellulase, 15 ： 1 V： W, 500 W and 30s microwave. The triple technology increases the content of SDF-P to （ 48.8 ± 0.03 ） % very significantly （p 〈 0.01 ）, 2.8 times as HC1- microwave or cellulase-mierowave treatment. Its yield is also over 11% , which increases by 1.7 and 2. 1 times. In summary, SDF-P can be extracted by triple technology efficiently by HCl-cellulase-microwave with lower industry cost.