T淋巴细胞疫苗诱导大鼠移植心存活时间延长的机制探讨

Study on the mechanisms of T cell vaccination-induced survival prolongation of cardiac allograft in rats

目的 探讨T淋巴细胞疫苗 (TCV)对大鼠移植心脏存活时间的影响及其机理。方法 取经供者抗原致敏的受者脾细胞 (1/3脾切除 )制备TCV ,然后将其回注于自身腹腔 ,观察大鼠异位移植心脏的存活时间。结果 接种TCV可明显延长大鼠同种移植心的存活时间 ;注射TCV后 ,T淋巴细胞增殖反应增强 ,B淋巴细胞增殖反应未受影响 ;混合淋巴细胞反应 (MLR)降低 ;脾细胞及TCV表型分析显示CD8+细胞增多 ;抗体介导的补体依赖性细胞毒作用无明显变化。结论 TCV接种是一个免疫刺激过程 ,其所介导的免疫低反应具有抗原特异性 ,可能与抗独特型反应增强有关 ;T淋巴细胞疫苗延长移植物存活时间的机制可能与体液因素无关。

Objective To investigate the effect of T cell vaccination (TCV) on the survival time of cardiac allograft in rat. Methods Rat heterotopic cardiac transplantation was performed. Donor antigen-primed recipients' spleen cells were obtained by 1/3 splenectomy for the preparation of TCV. TCV was then intraperitoneally into the abdominal cavity again. The survival time of cardiac allograft was observed. Results TCV could markedly prolonged rat cardiac allograft survival. T cell proliferation was increased and B cell proliferation was not affected in the vaccinated rats. The mixed lymphocyte reaction (MLR) was inhibited. The analysis of phenotypes on T cells showed that the number of CD8 + clones in vaccine cells and vaccinated rat spleen cells were increased. The antibody-mediated cytotoxicity was not affected by TCV. Conclusions TCV enhances the recipients' T cell-mediated immune response. The TCV-induced hyporeactivity to specific donor antigen is related to the induction of anti-idiotypic response and is not associated with humoral mechanisms.