提高裸鼠体内人胎肝细胞移植物存活率的方法探讨

Methodological exploration of improving the survival rate of human fetal hepatocytes transplanted into nude mice

目的 探讨人胎肝细胞移植入裸鼠体内能否存活及提高移植物存活率的方法。方法 将人胎肝细胞悬液分别移植至裸鼠的腹腔、肝脏、脾脏及肠系膜 ,3个月后进行移植部位的组织学观察。结果 仅移植入脾脏和肠系膜的组中各有 1只 (1/ 10 )受者可观察到存活的移植物 ,而移植入肝脏组无法区分裸鼠肝细胞和人胎肝细胞 ,其它组均未观察到移植物 ;移植后腹腔注射地塞米松 ,可将移植成功率提高到 6 / 10 ;若将人胎肝细胞采用球体细胞培养技术制成细胞球团行脾脏内移植 ,术后腹腔注射地塞米松 ,则移植成功率可达 9/ 10 ,术后 6个月仍可见存活的移植物。结论 人胎肝细胞移植到裸鼠体内至少可以存活 6个月 ;将移植物制成细胞球团 ,并联合应用地塞米松 ,可提高移植物的存活率。

Objectives To explore whether human fetal hepatocytes (HFH) transplanted into nude mice survived and study the methods for raising successful rate of transplantation. Methods The suspension of human fetal hepatocytes was transplanted into the abdominal cavity, liver, speen and mesenterium respectively. Three months later, histological observation was performed. Results The HFH could be found on the transplanting sites of spleen and mesenterium with only 10*!% successful rate of transplantation. Intraperitoneal injection of dexamethasone after transplantation could increase the successful rate of the transplantation to 6/10. Under this circumstances, if HFH in cellular sphere culture was used as transplantation material, successful rate of transplantation could reach 90*!%. Based on methods mentioned above, a lobe of liver in nude mice was resected, successful rate of transplantation could reach 100*!%,and HFH transplanted into the spleens of nude mice still survived 6 months after transplantation. Conclusions HFH transplanted in nude mice could survive for at least 6 months. If transplantation materials were made up into cellular sphere cultures,combining with using dexamethasone, the survival rate of transplantation might significantly be increased.