一株家鼠型肾综合征出血热病毒的分离鉴定及核苷酸序列分析

Isolation and identification of a Seoul type HFRSV and analysis for its nucleotide

目的 探讨保定地区肾综合征出血热 (HFRS)流行高峰的原因。方法 RT RCR法分型、病毒分离、鉴定及核苷酸序列测定。结果 31例急性期病人血清经RT PCR分型 ,2例为姬鼠型 (HTN型 ) ,2 4例为家鼠型 (SEO型 )。对其中 1例SEO型病毒进行分离、鉴定及其部分M基因的核苷酸序列测定 ,并与SEOUL、R2 2 两株病毒M基因核苷酸及氨基酸同源性比较分别为 :95 .15 %、93 .94%、97.2 7%及97.2 7%。结论 (1)所分离SEO型汉坦病毒为 1996流行高峰年的主要流行株。 (2 )未发现病毒基因片段的重排 ,基因重排变异似不是造成该发病高峰的原因。 (3)不同地区存在的SEO型病毒分子结构差异相对较小 。

Objective To study the cause of high epidemic of the HFRS in Baoding.Methods RT PCR was used to determine HV RNA from patients with HFRS.A Seoul type virus was isolated and its nucleotide sequence was analysed.Results In 31 serum samples,2 were found to be HTN type and 24 were SEO type.Compared with the prototype SEO virus strain and Chinese SEO virus R 22 strain,the homolgies of nucleotide sequence of the new isolation were 95.1% and 93.94% respectively,and that of the amino acid were 97.27% and 97.27% respectively.Conclusion The high epidemic of HFRS in 1996 was caused chiefly by SEO type virus,without evidence of reassortment of viral genes.SEO virus was relatively stable.