人参皂甙对CD_(34)^+造血干/祖细胞的增殖和分化作用

Effect of Ginsenosides on Proliferation and Differentiation of Human CD_(34)^+ Hematopoietic Stem/Progenitor Cells

目的 :探索人参皂甙 (GS)对CD34 +造血干 /祖细胞的刺激增殖和诱导分化作用。方法 :采用免疫磁珠法 (MACS)分离纯化脐血CD34 +细胞 ,在造血细胞半固体和液体培养体系中加入不同浓度的GS ,检测对CD34 +造血干 /祖细胞增殖 ,形成祖细胞集落的提高率 ,并用流式细胞仪检测液体培养后细胞表面标记的变化。结果 :GS( 5～ 50 μg/ml)能提高BFU E、CFU E、CFU GM、CFU GEMM集落产率 ( % ) ,分别为87 6± 2 6、63 3± 2 8、58 0± 3 1和 96 3± 5 5(均P <0 0 1)。GS 2 5μg/ml是液体培养刺激CD34 +细胞体外增殖的最佳浓度。GS诱导细胞 14天后 ,细胞表面表达CD33+细胞随GS的浓度升高而增加 ,在GS 50 μg/ml时以CD15+细胞数最高 ,CD71+细胞和G A+细胞数仅在 2 5μg/ml时高于未加GS的对照组。结论 :GS不但能促进CD34 +造血干 /祖细胞的增殖 ,并且能诱导定向分化 ,具有类生长因子和协同生长因子的作用。

To investigate the effect of ginsenosides (GS) on proliferation and differentiation of human CD 34 + stem/progenitor cells. Methods: CD 34 + hematopoietic progenitor cells were isolated from umbilical cord blood by using the immune beads sorting system. The cells were exposed to GS of different concentrations in both liquid culture and semi-solid culture, and the elevation rate on proliferation of CD 34 + stem/progenitor cells and colony formation were estimated. The cells were marked with monoclonal antibody and the marker was examined by flow cytometry after incubated with GS for 14 days. Results: GS (5-50μg/ml) could raise the colony production rate of BFU-E、CFU-E、CFU-GM、CFU-GEMM by (87 6±2 6)%、(63 3±2 8)%、(58 0±3 1)% and (96 3±5 5)% respectively (all P<0 01), and the best effect in improving cell proliferation of CD 34 + cells in vitro was obtained when the concentration of GS was 25 μg/ml. After incubation with GS for 14 days, number of CD 33 + cells was increased by GS in a dose-dependent manner with a peak increasing rate at 200 μg/ml. In the presence of GS 50 μg/ml, CD 15 + cells were reaching the peak. Number of CD 71 +and G-A + cells increased only when the concentration of GS was 25 μg/ml. Conclusion: GS could not only promote the proliferation but also induce the differentiation of CD 34 + hematopoietic stem/progenitor cells, GS may play the role by cooperating with hematopoietic growth factor, and by its growth factor-like function in the regulation of hematopoiesis.