胃蛋白酶合剂中胃蛋白酶效价的测定

Determination of pepsin valence in pepsin mixtrue

目的建立胃蛋白酶合剂中胃蛋白酶效价的测定方法。方法采用紫外分光光度法测定胃蛋白酶的效价,测定波长为275 nm,以酪氨酸为对照品,牛血红蛋白为底物。结果酪氨酸浓度在0.324 6～0.757 4 mg mL-1与吸光度呈良好的线性关系(r=0.999 8)。胃蛋白酶合剂所含的其他成分不干扰胃蛋白酶的效价测定。平均回收率为99.9%,RSD为1.0%。结论该方法简便、准确、可靠,可用于胃蛋白合剂中胃蛋白酶的效价测定。

Objective To develop a method for the determination of pepsin valence in pepsin mixture.Methods Ultraviolet spectrophotometry was applied to determine pepsin valence,and the detection wavelength was 275 nm.Tyrosine was the reference substance,and bovine hemoglobin was used as the substrate.Results There was good linearity at 0.324 6-0.757 4 mg mL-1（r = 0.999 8） for tyrosine.Other ingredients in the pepsin mixture did not interfere with the determination.The mean recovery was 99.9% with RSD of 1.0%.Conclusion The method is simple,accurate and reliable for the determination of pepsin valence in pepsin mixture.