宫颈癌标本中HPV16的早期基因E6、E7和LCR的测定分析

Analysis on HIV16 early genes E6,E7 and LCR detection in cervical cancer specimens

目的:分析宫颈癌标本中HPV16的E6,E7和LCR(long control region,LCR)的变异。方法:对125份HPV16阳性的宫颈癌标本利用PCR扩增HPV16 E6、E7和LCR片段,并对PCR扩增产物进行DNA序列测定。结果:在E6最常见的变异为T350G(100.0%),在E7区最常见的变异为T789C(87.5%),而在LCR最常见的变异为G7521A(91.1%)。此外,在E6发现一个新变异T527A,在LCR区发现4个新变异C13T,A7636C,C7678T和G7799A,而E7区高度保守,LCR变异的位点集中于YY1转录因子结合位点。结论:宫颈癌中HPV16存在E6、E7和LCR变异,对高危型HPV变异的分析有助于HPV的诊断和疫苗的设计。

Objective： To analyze the variations of HPV16 E6,E7 and long control region（LCR） in cervical cancer specimens.Methods： HPV16 E6,E7 and LCR segments in 125 positive HPV16 cervical cancer specimens were amplified,and the amplification products were detected by DNA sequencing.Results： The most common variation of E6 was T350G（100.0%）,the most common variation of E7 was T789C（87.5%）,and the most common variation of LCR was G7521A（91.1%）.In addition,one new variation of E6-T527A was found,four new variations of LCR-C13T,A7636C,C7678T and G7799A were found,the locations of LCR variations focused in binding sites of YY1 transcription factor.Conclusion： HPV16 E6,E7 and LCR variations exist in cervical cancer,analyzing high risk HPV variations is helpful to diagnosis of HPV and design of vaccine.