NF-κBp65、p-NF-κBp65在宫颈病变组织中的表达及意义

Expressions and significances of NF-κBp65 and p-NF-κBp65 in cervical lesions

目的:探讨宫颈病变组织中核因子NF-κBp65、磷酸化型NF-κBp65(p-NF-κBp65)的表达及临床意义,以及高危型人乳头状瘤病毒(hsHPV)对其表达的影响。方法:采用免疫组化SP法检测NF-κBp65、p-NF-κBp65在67例宫颈癌、149例宫颈上皮内瘤样病变(CINⅠ-Ⅲ)、15例正常宫颈组织中的表达。结果:NF-κBp65、p-NF-κBp65的表达随宫颈病变级别升高而升高,并与宫颈病变级别呈正相关(P<0.05);NF-κBp65、p-NF-κBp65在hs-HPV阳性组的表达高于hs-HPV阴性组(P<0.05);NF-κBp65、p-NF-κBp65在癌细胞高分化、肿瘤直径≤4 cm、临床Ⅰ-Ⅱ期组的表达低于癌细胞中低分化、肿瘤直径>4 cm、临床Ⅲ-Ⅳ期组(P<0.05)。结论:hs-HPV能促进NF-κBp65的表达和激活,NF-κBp65的表达和激活可能与宫颈癌的发生、发展密切相关。

Objective： To explore the expressions and significances of NF-κBp65 and p-NF-κBp65 in cervical lesions and the effect of high-risk human papillomavirus（hsHPV） on their expressions.Methods： Immunohistochemical SP method was used to detect the expressions of NF-κBp65 and p-NF-κBp65 in 67 cases of cervical cancer tissue,149 cases of cervical intraepithelial neoplasia（CIN） Ⅰ-Ⅲ and 15 cases of normal cervical tissue.Results： The expression levels of NF-κBp65 and p-NF-κBp65 increased with pathological grades of cervical lesions,which were positively correlated with degrees of cervical lesions（P 0.05）;the expression levels of NF κBp65 and p-NF-κBp65 in positive hs-HPV group were statistically significantly higher than those in negative hs-HPV group（P 0.05）;the expression levels of NF-κBp65 and p-NF-κBp65 in cervical cancer tissue of high differentiation,diameter of tumors ≤4 cm and stage Ⅰ-Ⅱ were statistically significantly higher than those in cervical cancer tissue of low-moderate differentiation,diameter of tumors 4 cm and stage Ⅲ-Ⅳ（P 0.05）.Conclusion： hsHPV can promote the expression and activation of NF-κBp65,which may be closely correlated with occurrence and development of cervical cancer.