马兰草抗炎活性部位量效关系及高效液相色谱-效关系研究

Dose-Response and HPLC Spectrum-Activity Relationships of the Anti-Inflammatory Active Site of Kalimeris indica

目的探索马兰草活性部位的量效关系及谱效关系。方法设置不同剂量组,观测抗炎活性之间的差异性,同时,测定不同剂量组的高效液相色谱指纹图谱,利用统计学方法,计算其图谱和药效之间的关系。结果不同剂量马兰草乙醇提取物与抗炎活性之间呈正相关性,相关系数为0.951 1;HPLC指纹图谱显示马兰草乙醇提取物有18个共有峰,指认了其中4个,各共有峰与抗炎活性之间的综合关联度在0.560 8～0.959 9之间,关联度大小顺序为:10>14>5>8>15>9>2>7>12>1>17>3>18>16>6>13>4>11。结论所建立的马兰草HPLC特征图谱与药效作用的关系,可为建立指纹图谱与药效关联的中药质量控制模式提供理论依据和数据支持。

ObjectiveTo explore the dose-effect and fingerprint-effect relationships of the active site of Kalimeris indica. Methods The HPLC fingerprints of the active site at different doses were determined. Mouse auricle swelling model was used to observe the anti-inflammatory action of Kalimeris indica extract and its dose-response relationship. Grey correlation method was used to assess the relationship between the fingerprint peaks and effects. Results The was a positive correlation between the dose and anti-inflammatory activity of the extract （r=0. 9511）. The extract had 18 common peaks, among which four were identified, and the comprehensive correlation between the common peaks and anti-inflammatory activity was from 0.560 8 to 0.959 9, with ranking of 10〉14〉5〉8〉15〉9〉2〉7〉12〉1〉17〉3〉18〉16〉6〉13〉4〉11. Conclusion The spectrum-activity relationship of Kalimeris indica established in this study could provide theoretical basis and data support for the quality control pattern of traditional Chinese medicines.