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结核杆菌临床分离菌早期培养滤液蛋白质组的比较 被引量:1

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摘要 目的:初步了解结核杆菌体外液培养条件下的早期蛋白质代谢和分泌活动状况。方法:将2株对抗结核一线药物敏感的结核杆菌临床分离茵接种Middlebrook7H9液体培养基进行培养,并于培养第7天用0.22μm滤纸过滤培养液制备早期培养滤液,应用与弱阳离子交换蛋白质芯片(WCX2)捕获滤液蛋白行表面增强激光解吸一电离飞行时间质谱(SELDI-TOF-Ms)检测,描述性分析比较其蛋白质组。结果:在2株结核杆菌7d培养滤液中分别检测到相对分子质量为982~9329与941。9331、丰度为7~210与11-368(通过质谱峰面积计算相对强度值表示)的164与194种蛋白质;与Middlebrook7H9液体培养基本身蛋白质组比较,在2株结核杆菌7d培养滤液中分别有153和183种蛋白质新产生(其中10种在2份滤液中均有存在)、92和90种蛋白质消失、10和13种蛋白质丰度变小。结论:在体外液体培养条件下,结核杆菌蛋白质代谢和分泌活动活跃。且不同菌株间有较大差异。
出处 《实用医学杂志》 CAS 北大核心 2013年第15期2456-2458,共3页 The Journal of Practical Medicine
基金 国家“十二五”传染病防治重大科技专项(编号:2012ZX10004-903) 广东省科技计划项目(编号:2011B31800377)
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