应用双水相萃取技术提取香菇多糖的研究

Extraction of Lentinan by Aqueous Two-phase System

利用聚乙二醇(PEG)-硫酸铵双水相体系提取香菇多糖,研究了不同PEG-硫酸铵组成的双水相体系对香菇多糖分配比和收率的影响。结果表明:PEG分子量在6000时对香菇多糖的提取效果较好;PEG6000浓度在24%时、硫酸铵浓度在30%时组成的双水相体系提取香菇多糖的效果最好,此时香菇多糖的分配系数(K)可达到1.90,收率(Y)可达到57.42%。

The use of polyethylene glycol （PEG） -ammonium sulfate aqueous two-phase system to extract lentinan was in- vestigated in this study. The effects of different PEG-ammonium sulfate aqueous two-phase systems on the partition coef- ficient and the extraction yield coefficient of lentinan were determined. The results showed that PEG with molecular weight at 6000 was suitable to extract lentinan and the aqueous two-phase extraction system eomposited of PEGr000 and ammonium sulfate at the concentration of 24% and 30% respectively can get higher distribution coefficient （1.90） and yield coefficient （57.42%） of lentinan.