我国4省肉鸡屠宰场沙门氏菌脉冲场凝胶电泳分子分型

Molecular typing of Salmonella isolates from poultry slaughterhouses in four provinces by pulsed-field gel electrophoresis

目的了解我国四省肉鸡屠宰场沙门氏菌脉冲场凝胶电泳分子分型情况。方法参照美国疾病预防控制中心PulseNet实验方法,对2010年监测4省肉鸡屠宰场分离到的167株沙门氏菌,运用XbaⅠ酶进行酶切并完成PFGE分析,利用BioNumerics软件对分离株的指纹图谱进行聚类分析并建立数据库。结果 167株沙门氏菌共分为18个群,包括了75个不同的带型。相同血清型具有相似带型,基本归于同一群。65株印地安纳沙门氏菌有38个带型,54株肠炎沙门氏菌有16个带型,16株奥尔巴尼沙门氏菌仅有1个带型。结论各省沙门氏菌的带型具有地区性差异,又具有优势型别的交叉。屠宰场存在严重的交叉污染,加强加工环节中的关键控制点—沙门氏菌的监测和干预,从而降低肉鸡及其产品中沙门氏菌的污染,这是从源头控制污染的根本措施。

Objective To study the pulse-field gel electrophoresis（PFGE）type of Salmonella strains isolated from poultry slaughterhouses in four provinces.Methods DNA collected from 167 Salmonella strains were digested by XbaI according to the standard PFGE protocol of US CDC.The PFGE patterns were analyzed with BioNumerics software.Results 167 Salmonella strains were clustered into 18 groups with 75 different patterns,and the same serotype had the similar PFGE pulse types which could be classified to one group.65 S.Indiana were clustered into 38 patterns.54 S.Enteritidis were clustered into 16 patterns.16 S.Albany were clustered into 1 pattern.Conclusion The distribution of pulse types of Salmonella isolates was different between regions,but some pulse types were cross-distributed.Cross-contamination was serious in slaughterhouses.Monitoring and intervention of critical control point during processing should be strengthened in order to decrease salmonella contamination in poultry products,and that was the basic measure of source control.