2009—2012年上海市副溶血性弧菌血清分型研究

Study on the serotype of Vibrio parahaemolyticus isolates during 2009-2012 in Shanghai

目的了解2009—2012年期间上海市副溶血性弧菌临床菌株和水产品分离株的血清型分布。方法采用血清玻片凝集试验对622株临床菌株和176株水产品分离株进行血清分型。结果 622株副溶血性弧菌临床菌株可分为27个血清型,其中以O3∶K6型为主,约占61.7%。176株副溶血性弧菌水产品分离株可分为9个O群,其中O1、O2、O3、O4和O5群为主要血清型,约占72.2%。水产品分离株与临床菌株的血清型差异较大,但在水产品中检测到2株临床菌株的优势血清型O3∶K6。结论 2009—2012年期间上海市副溶血性弧菌临床菌株和水产品分离株的血清型均呈现高度多样性,O3∶K6型是引起食物中毒的优势血清型,而水产品分离株未见优势血清型。临床菌株优势血清型O3∶K6在水产品中的出现,提示上海市水产品存在潜在的致病危险,需要加强监控,为疾病的预防和控制提供依据。

Objective Objective To study the serotype distribution of Vibrio parahaemolyticus clinical isolates and aquatic products isolates during 2009-2012 in Shanghai.Methods Using slide serum agglutination test to detect the serotypes of Vibrio parahaemolyticus from 622 clinical and 176 aquatic samples.Results Among 622 clinical isolates,27 serotypes were identified.The predominant serotype was O3∶ K6（61.7%）.It was shown that 176 aquatic strains could divide into 9 serum groups.The predominant serotype O3∶ K6 among clinical isolates was detected in two aquatic products.Conclusion The serotypes among clinical isolates and aquatic products showed high diversity.The predominant serotype O3∶ K6 among clinical isolates appeared in aquatic product representing a public health risk and requiring epidemiological and ecological monitoring to ensure safety.