白芷中欧前胡素和异欧前胡素的含量测定

Determination of Imperatorin and Isoimperatorin in Radix Angelicae Dahuricae

目的:建立白芷中欧前胡素和异欧前胡素的高效液相色谱含量测定方法。方法:色谱柱:Venusil XBP C18(4.6 mm×250 mm,5μm),以甲醇-水(60∶40)为流动相,体积流量1.0 mL.min-1,柱温:30℃,检测波长304 nm。结果:欧前胡素和异欧前胡素分别在0.15～1.80μg和0.10～1.20μg范围内呈良好的线性关系(r分别为0.999 5、0.999 6);平均回收率分别为100.2%、99.4%,RSD%分别为1.57、1.21(n=5)。结论:该方法简便、可靠、重现性好,可用于测定药材白芷中欧前胡素和异欧前胡素的含量。

Objective：To establish a method for the simultaneous determination of imperatorin and isoimperatorin in radix angelicae dahuricae by HPLC.Methods：The separation was performed on a Venusil XBP C18（4.6 mm× 250 mm,5 μm）,column using methanol-water（60：40）as mobile phase at 30 ℃,and the flow rate was 1.0 mL·min-1.The detection wavelengths was set at 304 nm.Results：The linear relation was in the rang of 0.15~1.80 μg（r=0.999 5）for imperatorin,0.10~1.20 μg（r=0.999 6）for isoimperatorin,respectively.Their average recoveries were 100.2%（RSD was 1.57%）,99.4%（RSD was 1.21%）.Conclusion：The method is simple,reliable,and reproducible for the content determination of imperatorin and isoimperatorin in radix angelicae dahuricae.