青皮内生菌Phomopsis sp.固体发酵产物的分离纯化

Extraction and Isolation of Solid Culture of Endophyte Phomopsis sp.

目的:依据内共生理论,从青皮内生菌Phomopsis sp.固体发酵产物中寻找与宿主植物相同或相似的药理活性成分。方法:利用硅胶柱层析、SephadexLH-20柱层析、反相柱层析以及高效液相色谱等方法对青皮内生菌Phomopsis sp.固体发酵产物进行分离纯化,并采用1 H-NMR谱、13 C-NMR谱和质谱等技术鉴定单体化合物的结构。结果:从组分QLB-SZP中分离获得一个单体化合物QLB-SZP-1(4mg)。结论:内生菌Phomopsis sp.产生的代谢产物结构类型丰富,可以为天然药物研究提供化合物来源。

Objective： To isolate bioactive natural product identical to or similar to host plant from solid culture of endophyte Pho mopsis sp. on the basis of endosymbiotic theory. Methods： Isolation and purification of solid culture of endophyte Phornopsis sp. was carried out through silica gel chromatography, Sephadex LH-20 gel filtration, ODS chromatography and HPLC, structure of the obtained compounds were elucidated by ^1H,^13 C-NMR spectra and mass spectra. Results ： Compound QLB-SZP-1 （4mg） had been obtained from component QLB SZP. Conclusion. Endophyte Phornopsis sp. could produce second metabolites with various struc ture, which provided sufficient compounds for research on natural drug.