犬腺病毒Ⅱ型弱毒疫苗株的分离、鉴定与筛选

DISCRETENESS,IDENTIFICATION AND RIDDLING OF CAV-ⅡHYPOTOXIC VACCINE

应用犬肾传代细胞 (MDCK) ,通过人“O”型红细胞吸附和氯仿处理等方法 ,从美国犬四联弱毒疫苗中分离出一株腺病毒 ,经形态、理化及生物学特性等系统鉴定证明 :该毒株是犬Ⅱ型腺病毒 ,命名为CAV2 XN3 株。实验将该病毒复制成弱毒疫苗 ,免疫 8只 2月龄当地幼犬 ,经临床观察证明 ,试验犬未出现不良反应 ;免疫后 14天采血测定血清中和抗体效价达到 1∶12 8,免疫后 2 5天血清中和抗体效价达到 1∶2 5 6 ,免疫后 10个月降至 1∶32 ,但仍能耐受强毒攻击 ,而对照组 4只犬则发病死亡。表明该弱毒疫苗激发免疫快 ,免疫期长 ,安全有效。

An adenovirus has been dissevered from American canine quaternity hypotoxic vaccine,with MDCK,hominine O type RBC adsorption and chloroform treatment.It is demonstrated CAV 2 adenovirus and named CAV 2 XN 3,after analysing the morphological,physical,chemical and biological features.The authors replicate the virus into hypotoxic and immunize 8 local young canines of two months old,without harmful reactivity being found.The neutralizing antibody titer of serum is 1∶128 14 days later,1∶256 25 days later,but still standing intensive poison with 1∶32 10 months later.Four canines of control group have died.The experiment shows the hypotoxic vaccine belongs to quick provocative immunity,long immunity period,and safe and effective as well.