滞留时间法评价促透剂对积雪草苷体外经皮释药特性的影响

Evaluation on the permeation of the penetration enhancers on asiaticoside by lag-time method

目的考察薄荷油、冰片和氮酮对积雪草苷体外经皮渗透的影响。方法使用改良Franz体外透皮装置，采用反相高效液相色谱法检测接收液中积雪草苷的浓度并计算药物的经皮累积渗透量，采用滞留时间法评价不同促透剂对积雪草苷的经皮释药特性的影响。结果氮酮对积雪草苷的经皮促透作用最好，24h内累积经皮渗透量为7．120μg／cm2,滞留时间适中为0．366h。1％冰片和1％的薄荷24h内累积经皮渗透量分别为5．025μg/cm2和4．660μg／cm2，滞留时间分别为0．195h和0．547h。结论氮酮、冰片均能够提高积雪草苷的透皮效率，薄荷油对积雪草苷的促透作用较差。

Objective To evaluate the transdermal absorption effect of the pennyroyal, borneoland azone on asiaticoside of vitro. Methods Tile modified Franz diffusion cell and excised rat skin in vitro as transdermal barrier was adopted in this experiment. The solution was determined by reversed-phase high perform liquid chromatography method. Lag-time method was used to acquire the correlation coefficient of percutaneous absorption. Results The transdermal permeation effect of azone was stronger than that of pennyroyal and borneol. The 24-hour cumulative permeation quantity of azone was 7.120μg/cm2 and the lag-time was 0.526 hour. The 24-hour cumulative permeation quantity of 1% borneol and 1% peppermint were 5.025μg/cm2 and 4.660μg/cm2, respectively, and the lag-time were 0.195 hour and 0.547 hoar, respectively. Conclusion Azone and borneo demonstrate greater effects on the transdermal permeation rate than pennyroyal.