杂交酶及其相关技术研究进展

Research advances in hybrid enzymes and relative techniques

根据杂交酶研究目的以及构建杂交酶方法，文中总结近年来杂交酶研究的成果；将杂交酶的应用分为以下几个主要的方面：改变酶的非催化特性；创造新活性的酶；研究蛋白质的结构和功能的关系等。还介绍了DNA序列改组、表达克隆、分子筛选、人造细胞样区室筛选基因等技术的进展以及这些技术在构建杂交酶方面的应用。

The research advances in hybrid enzymes have been summarized in this review according to the reseaxch purpose and construction methods. The hybrid enzyme techniques have been used to modify enzyme's non-catalysis characters, create novel enzymes, study the relationship between function and structure of protein. Meanwhile, DNA shufIling, expression cloning, molecular screening, and man made cell-like compartment techniques are aJso introduced.