苁蓉冲剂对白细胞减低症模型小鼠抗氧化功能的影响

Effect of Congrong Granule on Antioxidative Function in Leukopenia Mice

目的:探讨苁蓉冲剂对白细胞减低症模型小鼠抗氧化功能的影响,为扩大苁蓉冲剂的临床应用范围提供实验依据和理论基础。方法:采用环磷酰胺建立白细胞减低症小鼠模型,建模成功后,随机分组,用3个剂量苁蓉冲剂(0.7 g/kg、1.4 g/kg、2.8 g/kg)分别进行灌胃,持续经口灌胃给药15 d,小鼠摘除眼球取血,按试剂盒说明书分别测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的含量。结果:用药后,高剂量组(2.8 g/kg)小鼠较模型对照组小鼠血清MDA含量降低,SOD活力增高,差异有统计学意义;且高剂量组小鼠用药后与用药前相比,血清MDA含量降低,SOD、GSH-Px活力增高,差异也均有统计学意义。结论:高剂量苁蓉冲剂对白细胞减低症模型小鼠具有一定的抗氧化功能。

Objective： To investigate the effect of Congrong granule on antioxidative function in leukopenia mice and to provide experimental evidence and theoretical basis for the expansion of the clinical application of Congrong granule.Methods： Leukopenia mice models were made with cyclophosphamide,which were randomly divided into three dose groups（0.7 g / kg,1.4 g / kg,2.8 g / kg） and then given Congrong granule by gavage,continuously for 15 days.Enucleated blood of mice was taken to measure the contents of SOD,GSH-Px,MDA according to kit instructions.Results： Compared with the control group,serum MDA content significantly decreased and SOD activity significantly increased in high-dose group（2.8 g / kg）.Compared with those in the high-dose group before adminstrastion of Congrong granule by gavage,serum MDA content also significantly decreased and the activity of SOD and GSH-Px significantly increased as well.Conclusions： Congrong granule have antioxidative function in leucopenia mice and can be used as an adjunct to anti-tumor therapy.